Monday, January 27, 2020

The Problems With Ship Breaking In Bangladesh Environmental Sciences Essay

The Problems With Ship Breaking In Bangladesh Environmental Sciences Essay Introduction Environmental degradation and depletion of resources are alarmingly increasing in developing countries due to lack effective legislations, non-compliance of international standards in ship breaking process. It is manifested mainly by pollution, deforestation, destruction of wetlands, depletion of soil nutrients, etc. In Bangladesh, both rapid population growth and a degraded environment create serious threats to the economic development. This leads to other serious problems such as poverty, malnutrition and illiteracy. Since the majority of people in Bangladesh derive their livelihoods from the use and extraction of all types of resources, the living condition in Bangladesh, especially of the poor, becomes extremely vulnerable to environmental damage  [1]  .  The instant paper is directed to scan the relevant literatures on the impact of ship breaking in Bangladesh and also to put forward some proposals to be undertaken to this end. Super tankers and giant cargo ships are the backbone of our global consumer society. Hundreds of meters long, ferrying millions of tons of goods across the globe, the perpendicular size of these immense vessels is awe inspiring. Even when these ships are not seaworthy anymore, and repairs are not inexpensively viable, the raw material it is constructed from has a greater value for other purposes. Construction of one such behemoth is a fascinating feat of engineering, however, the destruction and final resting place of these steel giants is even more intriguing. Ship breaking is the process that dismantles an obsolete vessels structure for scrapping or disposal. Conducted at a pier or dry dock or dismantling ship, it includes a wide range of activities, from removing all gears and equipment to cutting down the ships infrastructure (OSHA, 2001)  [2]  . Ship breaking although is demanding but a very risky process indeed. It involves pollution, environmental hazards, and health issues. Ship breaking was recognized as a highly mechanized task until the 1960s in the industrialized countries like the United States, United Kingdom, Germany and Italy, but as the costs of upholding environmental health and safety standards increased the activity moved towards the poor Asiatic states from the early 1980s. The industry since then took a different approach where the ship owners to maximise profits sent their vessels to the scrap yards of poor South Asian countries like India, Bangladesh and Pakistan where payment, health and safety requirements are minimal and workers are desperate for work. Ship breaking activities in Bangladesh is concentrated on the port city Chittagong on the Bay of Bengal. Ship breaking activities are being practiced in the coastal areas and have gained importance in the macro and micro-economy of poverty stricken Bangladesh. Rising demand of raw materials for re-rolling mills and other purposes has made ship breaking activity as one of the major character in the industrial revolution of Bangladesh. Bangladesh is dependant on its ship breaking industry for its domestic steel requirements, thus making it as one of the major resources for steel. This is why ship breaking industry is not subject to any environmental laws or health and safety regulations for workers in Bangladesh. Even though there is no such law regarding ship breaking and on top of that no one is even concerned about the environmental hazard which is leading the country to an outrageous environmental exposure. After the vessels end their navigability period, the owners send them to the shipyards in Chittagong to recover their valuable steel where about 95% of a vessel is recycled. However, these ships contain hazardous substances such as asbestos, lead paint and polychlorinated biphenyls (PCB). During scrapping, by manual and basic methods, these toxic substances are released into the atmosphere. This phenomenon produces a strong and considerable contamination of the surrounding environment and lethal diseases into the workers. These activities exemplify both the potentialities and the dangers of an increasingly globalising economy.  [3]   PROBLEMS DUE TO SHIP BREAKING Ships built before the 1980s, contain many deadly materials. Ship breaking activities in Chittagong, Bangladesh pollute the soil, sea and rivers of the surrounding. Local fishing and agriculture as a livelihood are almost impossible over there due to the discharge of this toxic gases and oil pollution in the sea. This erosion of their traditional livelihoods drives more and more impoverished people to the dangerous shipyards. Samples taken from the yard shows contamination with high levels of mineral oil and toxic substances. Discharge of oil to the sea also results physical damage to the birds, mammals and marine organisms and their natural habitats. Wastes like blasting residue and paint chips contaminate the soil and surface water. Improper storage and disposal of scrap metals and wastes also causes lead contamination. Environmentally hazardous fumes evolve when metal and paint is heated during hot work. The pollution is both severe and long-standing. Unlike in Western countries w here there are stringent regulations on hazardous substances and health and safety measure, in Chittagong yard rules and regulations are rarely applied. Laws on health and safety matters although exists but are hardly enforced and moreover even if the law becomes stringent due to corrupt politics the ship owners apply their powers to withdraw the law and orders.  Following a High Court directive, the commerce ministry of Bangladesh incorporated a condition in its Import Policy Order 2009-2012, which said an exporter of a vessel had to submit a pre-cleaning certificate from the country of origin stating the ship was cleaned before exportation but then again the government amended the law allowing the Chittagong ship breaking industry to no longer require the documentation from the selling nations environmental authorities certifying vessels were free of toxic substances.  [4]  Ã‚  The Basel convention and several other international treaties laid down rules and regulations whic h states that ships that are built with deadly toxic substances shall not be exported for recycling unless they are pre-cleaned and has undergone a cleaning test. But in Chittagong ship breaking yard the owners in violation of the law import those toxic vessels without pre-cleaning. As a result of this violation, terrible poisons and toxic gases are released into the environment playing havoc with the health of the people and all living creature in the ecosystem. The workers in Chittagong yard are permanently exposed to toxic substances. They breathe in these toxic fumes and asbestos dust. Not only on the job, but also in the sleeping quarters nearby they inhale these toxic gases. One out of four workers is expected to contract cancer due to workplace poisons. This makes the Chittagong ship breaking industry the most deadly in the world. ENVIRONMENTAL HAZARDS DUE TO SHIP BREAKING Aside from the health of the yard workers, in recent years, ship breaking has become an issue of major  environmental concern. In addition to steel and other useful materials, however, ships contain many hazardous substances. Many ship breaking yards in developing nations have no  proper enforceable environmental law, enabling large quantities of highly toxic materials to escape into the environment causing serious health problems amongst the labours in the ship breaking yard, the local population and wildlife.  [5]  The hazards are as follows: Hazardous Substances Asbestos fibres, dusts PCBs PVC (combustion products) Heavy and toxic metals(lead, mercury, cadmium, copper, zinc etc. Welding fumes Organometallic substances (tributylin, etc.) Volatile organic compounds (solvents) Lack of hazard communication (storage, labelling, material safety data sheets) Inhalation in confined and enclosed spaces Batteries, fire-fighting liquids Compressed gas cylinders Physical Hazards Noise Vibration Extreme temperatures Poor illumination Biological Hazards Toxic marine organisms Animal bites Risk of communicable diseases transmitted by pests, vermin, rodents, insects and other animals that may infest the ship others) Infectious diseases (TB, malaria, dengue fever, hepatitis, respiratory infections, others) Ergonomic and Psychological Hazards Repetitive strain injuries, awkward postures, repetitive and monotonous work, excessive workload. Mental stress, strained human relations (aggressive behaviour, alcohol and drug abuse, violence) IMPACTS OF SHIP BREAKING DUE TO DISCHARGED POLLUTANTS Most hazardous materials nowadays are restricted or banned today but a ship built about 30 years ago still contains these materials. It also carries hazardous and flammable chemicals used for painting, repair and maintenance, etc. Cables and electrical and other control systems contain hazardous material and emit hazardous gases, if burned. The paint coat, contaminated air, soil and water when torched or scraped, is hazardous for human and the environment. The protection of health and of the workers handling the hazardous waste is of crucial importance.  [6]   Persistent Organic Pollutants (POPs) POPs are chemicals that are highly toxic, remain intact in the environment for long periods, become widely distributed geographically, bio accumulate through the food web, accumulate in the fatty tissue of living organisms and pose a risk of causing adverse effects to human population, wildlife and the environment.  [7]  This group of priority pollutants consists of pesticides such as DDT, industrial chemicals such as polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), polyvinyl chloride (PVC) and unintentional by-products of industrial processes such as dioxins, furans and orgaotins. POPs are transported across international boundaries far from their sources no matter wherever they are used, even to regions where they have never been used or produced. Due to its emission the ecosystems particularly becomes risky because of the long-range environmental transportation and bio-magnification of these substances. Consequently, POPs pose a threat to the environm ent and to human health all over the globe. Exposure to POPs may cause acute, medium or long-term impacts. It also gives rise to cancer and other related diseases. Chittagong ship breaking industry is a prospective basis of these fatal chemicals of POPs. Asbestos Asbestos is the name given to a group of six different fibrous minerals (Amosite, Chrysotile, Crocidolite, and the fibrous varieties of Tremolite, Actinolite, and Anthophyllite) that occurs naturally in the environment. All forms of asbestos are hazardous, and inhalation of these fibres can cause severe diseases. On the ship breaking beaches of Chittagong, asbestos fibres and flocks fly around in the open air and the labours over there take out asbestos insulation materials with their bare hands without any safety precautions. Workers who repeatedly breathe in asbestos fibres with lengths greater than or equal to 5ÃŽÂ ¼m may develop a slow build-up of a disease called asbestosis. a scar-like tissue in the lungs and in the membrane that surrounds the lungs. This is a serious disease and can eventually lead to disability or death in people exposed to high amounts of asbestos over a long period. Asbestos workers also have increased chances of getting lung cancer as well. Oil Pollution Oil is a highly volatile compound composed of mainly hydrocarbon, sulphur containing compounds etc. Approximately 75% of the constituents of oil are hydrocarbons. As a result ship breaking, oil residues and the other refuses are being spilled and mixed with soil and water in the beach. In Chittagong yard the beach is black due to oil spills, and fishes have already started to disappear as per the researchers report. In freshwater environments, perhaps the most dangerous problem is contamination of drinking water sources. Food sources, such as fish and crustaceans both marine and fresh water, may be tainted and the consumption of tainted food causes human health problems and also loss for export trade in foreign market. Oil pollution also threatens the livelihoods of fisherman. Impact of ship breaking on the surrounding environment and coastal community Toxic oil and metallic substances through intake of affected fish, causes human health hazards. In ship breaking areas various refuse and disposable materials are discharged and spilled from scraped ships and it often gets mixed with the beach soil .The scrap from the ships is staked haphazardly on the sea shore, leaving behind an accumulation of metal fragments and rust in the soil. These together with extensive human and mechanical activities often go on as routine work for the scrapping of ships in that area as a result the beach soil loses its binding properties and this accelerate the rate and the amount of shore erosion and increase the turbidity of sea water of the surrounding area. Disruption of bio-diversity in the long run may also destroy the suitability of human settlement. During the breaking period accidental death sometimes occurs. Due to lack of proper sanitation and drinking water workers suffer from water borne diseases. As the sound pollution is a regular phenomenon during dismantling, inhabitants of the adjacent areas live in a painful situation .The poor coastal community get a variety of employment opportunities in the industry and it turns into a way of livelihood to them. On the other hand social crime, abuse of drugs and illegal activities increases due to ship breaking activities in that area. As the commercially important species are replaced by low priced species and scarcity of fish, many coastal fishermen are leaving their hereditary profession and moving around everyday as environmental refugees in a state of under employment and poverty to unemployment and grim poverty. SHIP BREAKING AND INTERNATIONAL LAW The Rotterdam Convention on the Prior Informed Consent (PIC) (1998) PIC is internationally applied to the trans-boundary movement of hazardous waste. The Rotterdam Convention, subjects to the Prior Informed Consent procedure for 30 hazardous pesticides and 11 industrial chemicals such as chlordane, chlordimeform, chlorobenzilate, Asbestos, PBBs, PCBs etc. Most of these hazardous substances are present in ships that were built 20 to 30 years back. Countries lacking adequate infrastructure to monitor the import and use of such hazardous substances were in violence of the law. In terms of ship breaking, owners have to make a declaration of hazardous substances on board, on arrival at the yard for dismantling. However, PIC by itself is not sufficient to stop unsafe scrapping. What is needed is that the country from which the toxic ship arrives needs to notify the importing country. The importing country can then take measures for the adequate treatment of these hazardous substances. But unfortunately in the case of Chittagong ship yard it is often seen that end of life vessels have been exported without the importing authorities being alerted. The Basel Convention Articles 4 and 6 of the Basel Convention impose strict conditions that go further than the PIC convention on the trans-boundary movement of hazardous wastes. It imposes an obligation of due diligence, on all ship owners. All parties are required to provide information about a proposed trans-boundary movement of hazardous wastes, to the countries concerned. Three elements must be proved for the violation of the Basel Convention to the issue of ship breaking: (i) Proof that the waste will not be properly dealt with by the ship breaking country. (ii) The legal recognition that ships are waste. (iii) An established intention to discardby the owner of the ship.  [8]  This convention is very much applicable to the Chittagong yard but unfortunately they are hardly followed by both importing and exporting parties of the yard.  [9]   United Nations Environmental Program (UNEP) UNEP is responsible for the implementation of the Basel Convention. It is currently drafting guidelines on the environmentally sound management for the full and partial dismantling of ships, in order to provide recommendations on procedures and practices to be implemented to attain Environmental Sound Management (ESM) in ship breaking yards. International labour Organization (ILO) The International Labour Organization (ILO) is the UN specialized agency which seeks the promotion of social justice and internationally recognized human and labour rights. The guidelines suggest a national framework defining the general responsibilities and rights for employers, workers and regulatory authorities in ship breaking. In addition, the guidelines also provides recommendations on safe ship breaking operations including the management of hazardous substances, protection and preventative measures for workers against hazards and suggestions for a competency based training program. The ILO approach is to facilitate step-by-step improvements to the practice of dismantling ships on beaches. If these approaches are applied, the Chittagong ship breaking yard will reach a satisfactory quality.  [10]  The ILO guideline suggests the: Ensuring there is an inventory of hazardous materials on board; Decontamination and gas-freeing; Planning for safe demolition; Recycling and; Safe waste management. The International Convention for the Prevention of Marine Pollution from Ships (MARPOL) MARPOL is a combination of two treaties adopted in 1973 and 1978 respectively and updated by amendments through the years. It is the main international convention covering prevention of pollution of the marine environment by ships from operational or accidental causes. In the issue of ship breaking MARPOL would apply to the discharges that occur from ships sent for ship breaking, which take place within the territorial waters of the ship breaking country. Practically this means that once the ship is delivered to a ship breaking yard any discharge, notably of oil or oily mixtures will be prohibited under this convention. Such oily wastes must be retained on board or discharged to reception facilities in port.  [11]   International Maritime Organization (IMO) In December 2003, the IMO issued a document entitled Guidelines on Ship Recycling. The Guideline is urbanized to give advice to all relevant stakeholders in the recycling process. The Guidelines suggest practical measures for all stages of the ship recycling process. They are: à ¢Ã¢â€š ¬Ã‚ ¢ New ship and equipment design, in particular to minimize the use to hazardous substances and waste generation as well as to facilitate recycling and the removal of hazardous materials; à ¢Ã¢â€š ¬Ã‚ ¢ Preparation of a Green Passport for new and existing ships; à ¢Ã¢â€š ¬Ã‚ ¢ Selection of a recycling facility and preparation of a ship for recycling including a ship Recycling Plan and;   à ¢Ã¢â€š ¬Ã‚ ¢ Roles for primary stakeholders including flag, port and recycling states, the Basel Convention, the ILO and the shipping industry. The Guidelines seeks to give confidence to recycling as the best means of ship disposal. In general, the Guidelines take the view that the responsibility for environment and worker protection in ship recycling facilities must respite with the recycling feature itself and with the regulatory authorities of the country in which it operates. Nevertheless, it is noted that ship owners and other stakeholders also have a responsibility to address the issues involved. The International Chamber of Shipping (ICS) The aim of ICS is to encourage high standards of operation and the provision of high quality and efficient shipping services. It also strives for a regulatory environment which supports safe shipping operations, protection of the environment and adherence to internationally adopted standards and procedures regarding ship breaking. ICS also promotes properly considered international regulation of shipping and oppose unilateral and regional action by governments. ICS took the initiative to establish an Industry Working Party on Ship Recycling (IWPSR) on the 23rd of February 1999. The IWPSR established an Industry Code of Practice on ship recycling in August 2001. This Code of Practice is the basis for the IMOs own guidelines. It is aimed at the ship owners, and encourages for the present shipping companies to initiate and complete a programme to identify and record, as far as is practicable, on each of their existing ships, any potentially hazardous material, as well as to make every e ffort to minimize the amount of potentially hazardous materials on board of the ship and while recycling of ships. Stockholm Convention on Persistent Organic Pollutants (POPs) The Stockholm Convention (Article 3.2) restricts the import and export of POPs to cases where, for example, the purpose is environmentally sound disposal. It also requires that POPs may not be transported across international boundaries without taking into account relevant international rules, standards and guidelines (Article6.1). The Stockholm Convention requires Parties to take measures to reduce or eliminate releases of POPs from intentional production and use (Article 3), unintentional production (Article 5) and stockpiles and wastes (Article 6). Concepts of Best Available Techniques (BAT) and Best Environmental Practices (BEP) are to be further elaborated by the Conference of the Parties. Bangladesh being a signatory government of the convention must take measures to eliminate or reduce the release of POPs into the environment in the Chittagong ship breaking yard.  [12]   The Universal Declaration of Human Rights The issue of human rights in ship breaking yards came to the attention of the international community in the 1990s through the concerted actions of different non-governmental organizations, above all Greenpeace, trade unions such as the International Transport Workers Federation and the International Metalworkers Federation, and intergovernmental bodies, such as the International Labour Organization. The awful working conditions and the dangers to the health and safety of the workers, publicized by several investigative media reports, led to a public outcry, which in turn generated an international mobilization on the issue.  [13]   SOLUTIONS FOR A SAFER ENVIRONMENT Abide by the law All the international treaties and the international UN bodies must together act against unacceptable ship breaking practices. The yard owners and the workers must have the appropriate knowledge regarding the law, health and safety issues and they also have to be encouraged to abide by the law as requires. The government must take reasonable steps to make the law obligatory to abide by on the ship breaking yards. Stop dumping toxic ships The shipping industry is responsible for its own vessels. They should take immediate measures to prevent pollution of the environment and protect the workers that dismantle their ships. They can do that by decontaminating and gas-freeing their ship-for-scrap before export to developing countries.  [14]   Gas Free Certificates No ship recycling facility shall undertake any cutting or dismantling activity onboard a vessel until a ship has been tested for flammability and a Gas-Free for hot works certificate granted. This test must take place at the ship recycling facility just prior to any cutting or dismantling taking place. Prevent pollution The shipping industry is responsible for its own vessels. They should take immediate measures to prevent pollution of the environment and protect the workers that dismantle their ships. They can do that by decontaminating and degassing their ship-for-scrap before export to Asia. Governments Role The government of Bangladesh should formulate and implement a national policy and principles for safe and sustainable ship breaking after having consultation with relevant organizations, employers and workers. They should also include this sector under the ministry of industry defined by the Factory Act, 1965 and formulate a policy so that, workers rights and welfare; occupational safety health (OHP) could be ensured and it could be eco-friendly as well. Usage of Technological Renovation Ship-owners should gradually make their vessels cleaner by the usage of better technology machineries during the recycling process. They should provide their workers with more and more safe and precautionary machineries that do not risk the workers life. During maintenance and survey stops, hazardous materials should be replaced with clean alternatives. In addition, ship-owners should, together with shipbuilders and classification societies, commit to the development of clean ships to avoid future disposal problems.  [15]   CONCLUSION Though in Bangladesh, ship breaking is now considered as a formal industry by law but the activities in real are not at all formalized yet. There is no enforcement of the labour laws, no legal binding framework to ensure Bangladesh comply with international human rights and environmental commitments. Above all, there are no consolidated policies or strategies or guidelines in Bangladesh for ship dismantling and there is a serious lack of communication between the responsible Ministries. According to several researchers the whole coastal area in Chittagong is in high risk by pollution generated from the ship breaking activities. Toxic chemicals and hazardous materials are not managed in an environmentally sound way; the beach is black due to oil spills, and fishes have started to disappear. Also the ship breaking activities has impact on the human health, directly to the workers; it has impacts on the food chain, on physiochemical properties of seawater, inter tidal sediments and soil and above all the on the biodiversity . As Bangladesh is one of the largest countries in respect of ship breaking activities, the subsequent impact on the environment and violation of human rights in the ship breaking yards drew the attention of different concerned bodies around the world as well as within Bangladesh in recent years. If the ship breaking industry is to bring development in the country, the same shall also be applied to ensure minimization of pollution effect. A longer stretch along the seashore is in no way justified for continuation of this business. Rather a certain separate zone like a dockyard should be selected by the competent authority to be undertaken at the right time, before it is too late. Considering the vital role of ship breaking in national economy ship breaking can not be stopped. Rather a sustainable approach should be taken to minimize the negative consequences of ship breaking activities in our coastal zone.

Sunday, January 19, 2020

What’s Nature Got To Do With It? :: Writing Technology Essays

What’s Nature Got To Do With It? I am taking a course in writing technology, and the last thing you would imagine as a topic is how natural writing can or cannot be. Our teacher for this course had us come up with a writing project. We were asked to make twenty words or less using any tool that was natural and did not involve high technology. This means we could not use computers, paints, or markers. In discussing what we could use, the class quickly broke down the options. It appeared almost impossible. We got nothing. This is where begging and pleading for ideas came in to play, and perhaps our instructor is a little financially better off if someone offered him a bribe, who knows.. How could it be done? Why? What would we learn about such an unnatural task that would even relate to the topic writing technology? What I learned rather quickly was that these very questions were all apart of why the assignment was given. What at first seemed to be a pointless exercise really answered these questions in a profound way which I want to share with you today. My hope is that you too get a renewed appreciation for writing and it’s history. In the essay from Pencils to Pixels, Dennis Baron details the world’s journey from the use and making of the pencil to the computer. Barron states that the pencil wasn’t originally intended to be used as a writing device. There’s a bit of information you probably hadn’t heard before. Yes, pencils were actually adopted as a tool by â€Å"note takers.. ..scientists...and others who need to write†. They were taken from artists and adapted it for use as a writing tool ( Dennis Barron 44). And so, in engaging in my project, I found myself thinking of how I could adopt a natural tool and adapt that tool to my writing task. I first thought about writing some letters in water, but I could really find no natural container to hold the water. I could have used some sticks or some type of colored liquid and take a snapshot of it, but there was no natural platform to hold the water. This was not going to work.

Friday, January 3, 2020

Essay on Pride and Perception in Pride and Prejudice by...

Pride and Perception in Pride and Prejudice by Jane Austen Jane Austens society values impressions, and considers them an important aspect of their culture. A first impression determines the entire perception of that person. In Pride and Prejudice, Elizabeth Bennet learns a hard lesson by basing her perception of other characters completely on their first impressions. The comedy is concerned with a heroine who must be educated out of a condition of self-deception brought on by the shutters of pride into a condition of perception when that pride had been humbled through the exposure of the errors of judgement into which it has led her (Watt, 98). Through occurrences within the novel Pride and Prejudice, the perception based on†¦show more content†¦She begins to take a general interest in their friendship, and in turn her opinion of Darcy becomes more atrocious. Elizabeth learns that all his [Darcys] actions may be traced to pride; and pride has often been his best friend (Austen 71). Wickham continues his conversation and eve ntually tells Elizabeth that Darcy threw him from his household into a life of poverty. Most important, of course, is Elizabeths misjudgement of Darcys character: the overreaction to his pride and reserve that makes her unable to see what lies beneath it (Moler, 26). Wickhams actions and words lead Elizabeth to a harsh perception of Darcy and a kind perception of himself. This same perception of Darcy eventually leads her to confront him about his abuse of Wickham. He has been so unlucky as to lose your friendship, and in a manner which he is likely to suffer from all his life (Austen 80). At this point Elizabeths original perceptions of both Darcy and Wickham reach their climax, and slowly begin to decline. Now, the second perception of Darcy and Wickham begins to alter the first. A letter written to Elizabeth by Darcy reveals the real intentions of Wickham. Mr. Wickhams chief object was unquestioningly my sisters fortune, but I cannot help supposing that the hope of reveng ing himself on me was a strong inducement (Austen 172). After discovering this information Elizabeth slowly ceases her relationship with Wickham. The major ‘action of theShow MoreRelatedComparative Study: Letters to Alice and Pride and Prejudice1502 Words   |  7 Pagesportrayed in Pride and Prejudice are creatively reshaped in Letters to Alice. The two texts, Letters to Alice and Pride and Prejudice, mirror and contrast the central values shared and explored by evaluating them; presenting them against Jane Austens context and that of Fay Weldon. 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All of Jane’s older brothers except for one and her younger brothers followed their father’s footsteps and became clergymen. Her younger brothers, Francis and Charles, â€Å"were trained at the Royal Naval Academy at Portsmouth, and became admirals† (Kelly). At the age of twelve or thirteen Jane started to write short storiesRead MorePride And Prejudice And Jane Eyre1681 Words   |  7 PagesIntroduction: Jane Austen and Charlotte Bronte explore social class in a number of different ways throughout their novels Pride and the Prejudice and Jane Eyre. They do this through the use of stylistic devices which in turn appeals to their different audiences. Both Jane and Charlotte are notable writers for their remarkable texts. 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Thursday, December 26, 2019

The Value Of Leadership In Oedipus Rex By Sophocles

Based on evidence, Athenians valued bravery, confidence and heroic strength in their leaders. For example, the entire reason Oedipus from Oedipus Rex by Sophocles became king of Thebes is because he defeated the Sphinx that was guarding the city, saving the citizens (info from the backstory). On page 11, a senator makes the comment, â€Å"if his bosom holds a grain of fear, curses like yours he never will abide.† The reader can assume the Athenians valued bravery because the senator makes a comment against the idea of fear. Also, Tiresias is mentioned to be â€Å"above all men,† which indicates that leaders need to be better than their inferiors (page 11). The king, the leader of Thebes, is referred to as â€Å"the mightiest head,† which signifies the†¦show more content†¦The outcome of dramatic impact is emphasized by the focus of one main subject. Oedipus attempts to escape him fate by running away, only to fulfill it (background information). Every ot her occurrence serves only to amplify the effect of the main subject. An example of this is the suicide of Jocasta, who hangs herself as a result of finding out she married her own son (page 44-45). This action emphasizes the fact that Oedipus attempted to avoid his fate, which made the consequences worse. In conclusion, rather than limiting the dramatic effect of the play, Aristotle’s three unities accentuate how the drama unfolds in the play and how it affects the listeners/readers. The whole idea of sight vs. blindness in Oedipus Rex points to the theme of fate and free will. For example, Tiresias points out that Oedipus â€Å"(has his) sight, and (does) not see,† referring to the fact that the king ignored his fate, instead, choosing to go out on his own (page 15). Oedipus is famously â€Å"blind† up until page 45, when he literally goes blind. Before he actually goes blind, the main character is â€Å"blind in (the) mind,† which means he is unable to see his fate that is right in front of him (page 14). By being blind to his own fate, Oedipus falls into the theme of illusion vs. reality. He lives in an illusion of perfection, being the king and married to a woman with whom he had four children (backstory). Reality hit Oedipus when it is made clear to him that he actuallyShow MoreRelatedKings as Main Characters in Literature Essay765 Words   |  4 Pagesto others. However, differences in culture, social system, values and social backgrou nd etc. actually endows heroes with diverse personalities varying from person to person and culture to culture. With rich imagination, every child molds his or her supreme leaders with authorities. But as time goes by, children are gradually matures, leaving their imagination during the childhood in the oblivious world, and their cognition of leadership also develops and changes without only confining to the worshipRead More Oedipus the King: Does Oedipus Satisfy the Definition of a Good Man?1415 Words   |  6 PagesDoes Oedipus Satisfy the Definition of a Good Man?      Ã‚  Ã‚  Ã‚   As a young man, Oedipus learned of his fate to kill his father and marry his mother.   Oedipus flees to a distant land to escape his terrible fate and inadvertently fulfills the prophecy. Unknowingly, Oedipus kills his father and enters the bed of his mother.   Was Oedipus was a good man who happened to suffer an unfortunate fate, or was he a truly bad person, whose fate was only just?   If we accept the Aristotelian views of good and badRead MoreCompare and Contrast Othello and Waiting for Godot Plays Essay1104 Words   |  5 Pages4/25/13 Oedipus the king is a representative of ancient Greek drama by Sophocles. The Latin title of the play is Oedipus Rex. Though produced as the second play in the three sequences of Theban plays by Sophocles, it finds its way at the top of the sequence as a result of internal chronology. The next play in the sequence is Oedipus at colonus and finally Antigone. The play is an excellent example of Greek tragic plays (Bloom, 51). Waiting for Godot is a play by Samuel Beckett, an Irish who wonRead MoreFall from Grace: Satan as a Spiritually Corrupt Hero in Miltons Paradise Lost2859 Words   |  12 PagesEnglish Dictionary defines a hero as: ol li value=1 A man distinguished by extraordinary valour and martial achievements; one who does brave or noble deeds; an illustrious warrior. li value=2 A man who exhibits extraordinary bravery, firmness, fortitude, or greatness of soul, in any course of action, or in connexion with any pursuit, work, or enterprise; a man admired and venerated for his achievements and noble qualities. li value=3 The man who forms the subject of an epic; theRead MoreDeveloping Management Skills404131 Words   |  1617 Pages3 THE CRITICAL ROLE OF MANAGEMENT SKILLS The Importance of Competent Managers 6 The Skills of Effective Managers 7 Essential Management Skills 8 What Are Management Skills? 9 Improving Management Skills 12 An Approach to Skill Development 13 Leadership and Management 16 Contents of the Book 18 Organization of the Book 19 Practice and Application 21 Diversity and Individual Differences 21 Summary 23 SUPPLEMENTARY MATERIAL 24 Diagnostic Survey and Exercises 24 Personal Assessment of Management

Tuesday, December 17, 2019

Stigma and Prejudice in the Workplace - 844 Words

Workplace contributes strongly to the wellbeing of an individual. This is because it provides people with opportunities that make the feel productive and achieve their potentials. However, stigma and prejudice can make the workplace to be a very stressful and results into unwanted problems such as anxiety and depression (Reichert, 2011). Therefore, this paper aims at discussing how to create a safe workplace for stigmatized individuals. The paper describes various approaches that can be implemented to make workplace safe and free from stigmatization. Various approaches that can be used to create a safe workplace environment reduce stigmatization. The first one is to ensure that the workplace has inclusive environment and place that do not seclude or exclude stigmatized individuals. This is to prevent exposing the individuals to contradictory environments, which might identify them with the problem, challenge, or uniqueness (Saleebey, 2009). According to Szeto and Dobson (2011), obvious environment and places at work places, such as having separate offices for alcoholics, mentally challenged, or different social places for homosexuals may further enhance discrimination of the individuals. This implies that work place environment should be designed as inclusive as possible. Spagnolo, Murphy, Librera (2008) reported 80% of homosexual individuals find it easy to work in places, where they are not separated during social events, such break time sessions. Therefore, workplacesShow MoreRelatedLeadership Prejudices of Men and Women in t he Workplace Essay1592 Words   |  7 Pagesperspective to contrast the different leadership prejudices of men and women in the workplace. The age-old debate of inequality for women in executive position will now be examined through online periodicals that show gender stereotypes in the workforce, different strategies men and women use to break the glass ceiling of the workplace, a focus on the interactions and reactions of a male Chief Executive Officer (CEO) versus a female CEO, leadership prejudices among women and women, and various studiesRead MoreBeing Part Of A Stigmatized Group1287 Words   |  6 Pagessexual stigma to describe this phenomenon in relation to sexual minorities. Sexual stigma, in the researcher’s terms, means that there is an inferior status in relation to a society that does not tolerate of any nonheterosexual behavior, identity, or relationship (Herek Garnets, 2007). An example of this stigma would be a religion frowning upon a homosexual relatio nship and not tolerating or devaluing the homosexual couple. Herek also describes this at an individual level: sexual prejudice meansRead MoreChildren With Disabilities And Disabilities Essay1276 Words   |  6 Pages stigmatization, and prejudice among other forms of challenges. As such, it is possible to see many people with disabilities (e.g. visible: wheelchair bond or with cane and invisible: mental health or learning disabilities) are being socially and culturally affected by the general population’s perspective on them. This paper discusses stigmas, barriers, prejudices, and discrimination as the major difficulties that people with disabilities face in college and in the workplace along with policies andRead MoreEssay on Impact of Stigma of HIV-AIDS in the South African Workplace1497 Words   |  6 Pagesâ€Å"Impact of Stigma of HIV/AIDS in the South African workplace.† This falls under a discipline of business management in the financial management degree I am currently doing and one of the key areas of focus within business management is workplace challenges. I believe that HIV/AIDS stigmas are challenges regularly faced in the South African work environment. The purpose of this research review is to gain insight and deeper understanding of what workplace stigma towards HIV/AIDS is in the workplace fromRead MoreEffects On Psychological Health When Labelled With Chronic Illness1022 Words   |  5 PagesImpact on psychological health when labelled with chronic illness eg. stigma So my topic was how psychological health was affected when someone was labelled with a chronic illness. More specifically stigma associated with chronic illness and how it could impact Ivan due to his conditions. When someone is suffering or living with a chronic illness it can have a huge impact on them psychologically and socially. Chronic Illness is a condition that is prolonged in duration, usually more than 3 monthsRead MoreThe Stigma Of Invisible Disabilities. Invisible Disabilities1650 Words   |  7 PagesThe Stigma of Invisible Disabilities Invisible disabilities are exactly what they seem to be: hidden from view. Invisible disabilities come in almost endless varieties, including mental illnesses, HIV, AIDS, diabetes, learning disabilities, epilepsy, and more. While there may be visible components to these disabilities, all of them can be virtually invisible and â€Å"hidden† from view. People often make assumptions based on what is visibly seen, so when someone is suffering from something invisibleRead MoreAlicia Kate O. Borja. English 27 A. Andre Dominic Peralta.1260 Words   |  6 PagesAlicia Kate O. Borja English 27 A Andre Dominic Peralta Reducing Stigma in Mental Health Conditions Background on Mental Health Mental Health is often used as a representation of mental health conditions such as anxiety disorders, depression, schizophrenia, and others. According to the World Health Organization (2016), mental health is a state of well-being wherein an individual has the capacity to realize his or her own potential, can manage with the normal stresses of life, can work efficientlyRead MoreStereotypes in Raymond Carvers Cathedral Essay1335 Words   |  6 PagesHe treats Robert in a negative manner at first relying on those prejudices, but as he comes to know Robert, he re-develops his stereotypes and interacts with Robert in a more positive way. Dr. Munyi of Kenyatta University states in â€Å"Past and Present Perceptions Towards Disability: A Historical Perspective† that fear, ignorance, and superstition are among many social factors that have led to the development of stereotypes and prejudices towards disabled people. Throughout history people with disabilitiesRead MoreThe Differences Between Mental Health And Mental Illness Essay1319 Words   |  6 Pagesillness. Education is essential to our country, workplace and schools; we can learn from one another, but first we must have an understanding about ourselves as individuals. Social Workers bring unique knowledge and skills to the table and are instrumental in furthering the profession. Education to outside cultures and social groups can help social workers understand the differences within the world and helps dismiss negative stereotypes and prejudice around different groups. Education is key to understandingRead MoreThe Impact Of Workplace Diversity On The Work place997 Words   |  4 Pages Workplace diversity is a term which describes the inclusion of people in the workplace regardless of their cultural differences such as gender, race and sexuality. Rather than disregarding them, diverse workplaces utilise these differences to widen the range of experiences and skills among their employees as stated by the Australian Breastfeeding Association (2012). Some professions, however, are more diverse than others. According to the Diversity Inclusion: Unlocking Global Potential - Global

Monday, December 9, 2019

Physical Understanding System Development -Myassignmenthelp.Com

Question: Discuss About The Physical Understanding System Development? Answer: Introduction: The roadmap in the business change is considered as the high level and graphical overview of the project deliverables and goals represented through a timeline (Becker et al. 2012). The roadmap shows the changes in the business along with a plan to implement those changes. The changes in the business mainly happens due to implementation of information and communication technology or change in the business objectives. The report is based on the CC Musics software selection and implementation plan. The software selection process is done based on the objectives of the business process change. The project needs to be small and budget friendly. The recommendations is a great source of su9ggesting various implementation considerations. Current Situation of CC Music: CC Music is currently doing business with one Northern Soul band. The owners are experienced musicians and music technology teachers. As the owners have a long history associated with the bands, they have found it possible to conduct events and manage bands. The organization is responsible for managing studio works, TV and Radio programs and band performing on parties and events. The organization currently using a website to publicize the band. The booking process is done over the phone. The postal system is used for receiving cheque and contracts. All the processes of CC Music is done manually based on paper work. Only two members that are Chris and Clive has to do all the works on their own. Issues with the current situation: As the organization does not have any technology to assist in the business activities there are various issues in the organization. The paper based works are often tend to error and if a paper is lost then the data will have to be collected, unless a copy is present. As the organization is looking forward to grow its business, the biggest challenge will be maintaining all the paper based work and managing data. Chris and Clive will not be able to handle the huge amount of work if more bands are registered. The communication is a key factor in business growth. The organization does not have any proper way of storing the conversation. The communication among the external factors over the telephone will surely be a serious issue while the organization will handle multiple tasks simultaneously. The reports have to be generated manually which is always full of errors and affect the decision making process badly. Roadmap: Business Process Change Objectives: CC Music is willing to adopt a standard business approach to grow its business. The change must make the organization able to provide better and quality services to various bands. Better tracking of data is another objectives of business process change. Timeline: Process Start Date End Date Identify the software solution options 02/05/18 02/08/18 Compare and Select the software solution 02/09/18 02/10/18 Identify software development methodology options 02/12/18 02/13/18 Select the methodology 02/14/18 02/14/18 Employ the team members 02/15/18 02/19/18 Develop system 02/21/18 03/20/18 Train User 03/01/18 03/06/18 Proposed Changes: The main factor in the change of the business process is implementation or integration of ICT system in the environment of CC Music. The organization wants to provide services to several bands. In order to that CC Music must improve its infrastructure and business process. The most effective change in the process is communication. The organization will be integrating a software solution that will be consisting of website so that the clients and audiences can communicate with business anytime. The next big change is the change of paper based work into digital automation. The proposed software will store all the data into the system and these data will be used for creating report. Till now there is no decision making support to the owners of CC Music. Through the implementation of the IS, CC Music will introduce the decision making support to the business environment. Potential Risks: The change will be inviting various risks that has to be identified and mitigate. The risks of the change are wrong project estimation, cyber threats, improper training, internal threats, additional cost to implement change and wrong software selection. Risk Name Impact Likelihood Mitigation Strategies Wrong project estimation Medium High Risk avoidance Cyber threats Extreme Medium Risk transfer Improper training Medium Medium Risk avoidance Internal threats (Farkas et al. 2016) Medium Low Risk limitation Additional cost to implement change High High Risk acceptance Wrong software selection Extreme Medium Risk avoidance Selecting the Software Solution: The software selection is a crucial part of the project planning. There are four choices in the hands of Chris and Clive. They can either go with the open source ERP or implement a information system through open source language and database. In both the cases, the cloud system is preferable as it will reduce cost of hardware and other in-house development charges. As per the aforementioned objectives, a system that can cope with the business growth and provide a good opportunity to CC Music to increase client base is preferable. The PHP and MySQL based system is suggested to CC Music as this software solution will allow Chris and Clive to customize and change the software as per business requirements easily and with low expense. Process of Implementation: The process of implementation is followed by a planning phase where the SDLC methodology is selected. The traditional waterfall model is best suited for this software development project as the project is small and straight forward. The requirements of the software is well known and the user requirement collection procedure will be significantly small. After the planning is completed, the project team members will be hired (Laudon and Laudon 2016). Hiring employees is the only major expense in this project. The system requirements will be collected and evaluated in the next phase. After the requirements are identified, the system design will be executed. In this phase, the system interaction, behavior, data flow, database design and many more activities will be done. Based on the designs the next phase, development, will be carried out. The developed system will be continuously tested until a functioning system is generated (Putri and Rosa 2016). The tested system will be deployed in the environment of CC Music in the deployment phase. The users of the system will be provided training on how to use it during testing phase. Conclusion: Form the above report it can be concluded that the system is a crucial factor in the business process change. Chris and Clive needs to support the project from the beginning and communicating with other stakeholders is important part. The ERPNext could be selected for the organization but as Chris and Clive are looking for a long-term system that can support business objectives, the PHP and MySQL based system is perfect. The organization can later modify the systems each and every aspect as per their requirement. Recommendations: Skilled IT Professionals: Chris and Clive must hire skilled IT experts so that no issue can occur during system development. It has been seen that many SME has faced issues as the developers did not have enough knowledge and skill. Communication with Stakeholders: Communication among the project team and stakeholders of CC Music have a significant impact on the project. The IT system development is a critical task and often face unexpected challenges. In such situations, the stakeholders must support the project but it is recommended that communicating with the stakeholders on periodical basis helps in gaining their trust. References Alavi, M., 1984. An assessment of the prototyping approach to information systems development. Communications of the ACM, 27(6), pp.556-563. Becker, J., Delfmann, P., Eggert, M. and Schwittay, S., 2012. Generalizability and applicability of model-based business process compliance-checking approachesa state-of-the-art analysis and research roadmap. Business Research, 5(2), pp.221-247. Briggs, J.A., Wolvetang, E.J., Mattick, J.S., Rinn, J.L. and Barry, G., 2015. Mechanisms of long non-coding RNAs in mammalian nervous system development, plasticity, disease, and evolution. Neuron, 88(5), pp.861-877. Chavan, M.M. and Dongre, Y.V., 2015. MULTI-NODE DATABASE APPLICATION DEPLOYMENT ON LAMP ARCHITECTURE. International Journal of Advances in Engineering Technology, 7(6), p.1721. Delorme, A. and Makeig, S., 2004. EEGLAB: an open source toolbox for analysis of single-trial EEG dynamics including independent component analysis. Journal of neuroscience methods, 134(1), pp.9-21. Dolibarr, 2., January, N., DESCHAMPS, R., december), D., released, D., available, D. and CRM, D. 2018. Open Source ERP CRM software - Dolibarr. [online] Dolibarr.org. Available at: https://www.dolibarr.org/ [Accessed 4 Feb. 2018]. Erpnext.com. 2018. Open Source Cloud ERP - ERPNext. [online] Available at: https://erpnext.com/ [Accessed 4 Feb. 2018]. Farkas, D., Paz, E., Garrison, E., Greene, J., Gonzalez, J. and Zhao, K., 2016. A Research Inventory Database for the Hudson/Mohawk River Watershed Project. May-2016. https://csis. pace. edu/~ ctappert/srd2016/2016PDF/a7. pdf, accessed November. Garcia, C.M., Abilio, R. and Malheiros, N., 2015. Approaches and Technologies for Systems Integration: A Case Study at the Federal University of Lavras. Revista de Sistemas de Informao da FSMA, (15), pp.11-22. Garcia-Zapirain, B., de la Torre Dez, I., Sainz de Abajo, B. and Lpez-Coronado, M., 2016. Development, technical, and user evaluation of a web mobile application for self-control of diabetes. Telemedicine and e-Health, 22(9), pp.778-785. Heise, H., Crisan, A. and Theuvsen, L., 2015. The poultry market in Nigeria: market structures and potential for investment in the market. International Food and Agribusiness Management Review, 18, pp.197-222. Johansson, B. and Sudzina, F., 2008. ERP systems and open source: an initial review and some implications for SMEs. Journal of Enterprise Information Management, 21(6), pp.649-658. Laudon, K.C. and Laudon, J.P., 2016. Management information system. Pearson Education India. Liu, H., Jezek, K.C. and Li, B., 1999. Development of an Antarctic digital elevation model by integrating cartographic and remotely sensed data: a geographic information system based approach. Journal of Geophysical Research: Solid Earth, 104(B10), pp.23199-23213. Maruthi, R. and Jayakumari, C., 2014. SMS based bus tracking system using open source technologies. International Journal of Computer Applications, 86(9). Matthews, M.S., 2015. PHP and MYSQL Web Development: A Beginner's Guide. McGraw-Hill Education. Mejabi, O.V., Azeez, A.L., Adedoyin, A. and Oloyede, M.O., 2015, May. Challenges to Open Data Institutionalisation: Insights from stakeholder groups in Nigeria. In Open Data Research Symposium. Odoo S.A. 2018. Homepage. [online] Available at: https://www.odoo.com/ [Accessed 4 Feb. 2018]. Oon, R.D., 2010. Open Source ERP. ISBN-13, pp.978-9673490226. Putri, T.P. and Rosa, P.H.P., 2016. Decision Support System to Choose Digital Single Lens Camera with Simple Additive Weighting Method. Scientific Journal of Informatics, 3(2), pp.167-176. Rosemann, M. and vom Brocke, J., 2015. The six core elements of business process management. In Handbook on business process management 1 (pp. 105-122). Springer Berlin Heidelberg. Sherman, G.G., Lilian, R.R., Barron, P., Candy, S. and Bhardwaj, S., 2014. Laboratory information system data demonstrate successful implementation of the prevention of mother-to-child transmission programme in South Africa: prevention of mother-to-child transmission-Progress towards the Millennium Development Goals. South African Medical Journal, 104(3), pp.235-238. Smets-Solanes, J.P. and De Carvalho, R.A., 2003. ERP5: a next-generation, open-source ERP architecture. IT professional, 5(4), pp.38-44. Tor, I.E., Iheanacho, A.C. and Okeke, A.M., 2017. Analysis of Socioeconomic Challenges of Using Local Storage Systems for Root and Tuber Crops in Benue State, Nigeria. Vidgen, R., 2002. Constructing a web information system development methodology. Information systems journal, 12(3), pp.247-261. Yuan, X., Wood, E.F. and Ma, Z., 2015. A review on climate?model?based seasonal hydrologic forecasting: physical understanding and system development. Wiley Interdisciplinary Reviews: Water, 2(5), pp.523-536.

Monday, December 2, 2019

Microbiology Unknown Lab Report Essay Example

Microbiology Unknown Lab Report Paper Preparing for class Day I Read in your lab manual about the following agar mediums: Blood Agar (pug 168), EMBED Agar (pug 170), Imitation Salt Agar 172)), McCracken Agar (pug 174), ND PEA Agar (pug 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to else red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them? Beta hemolytic, which is the complete lysine of red blood cells and hemoglobin. This results in complete clearing tooth blood around the colonies. Alpha hemolytic refers to the partial lysine of red blood cells and hemoglobin, This results in a greenish-grey disconsolation tot the blood around the colonies. Employees, sometimes called gamma hemolytic results in no change in the medium. 3. What color is the EMBED agar? Dark blue colonies with green metallic sheen or pink. 4, What does the EMBED agar select for? Cram-negative bacteria. 5. What bacteria can easily be differentiated on EMBED agar? Gram-positive. How is it recognized? It contains the dyes eosin and methyl blue, which inhibit the growth of gram-positive bacteria. 6. What color is the Imitation Salt Agar (MS)? Yellow color change in surrounding media. 7. What does the MS agar select for? It contains 7. 5% sodium chloride, which selects for organisms that re halogenated. 8. Which bacteria (that we have learned about in lecture) can be seen on MS agar? The organism Staphylococcus erasures. How can it be recognized? Capable Of imitation fermentation, signified by the color change in the surrounding media. 9. What color is McCracken Agar? We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Neutral red, and produce a pink color. 10. What does the McCracken agar select for? Gram. Negative bacteria. 11. What color is PEA agar? Small transparent colonies 12. What does the PEA agar select for? Isolation of obligate anaerobic bacteria. Class day 1 14 We will be setting up the Selective/Differential Media plates for each group s follows: Each group will have one bacteria sample and will do streak plates on each of the 3 agar plates (EMBED, PEA and McCracken). Label the bottom of the plate with your culture name. Invert your plates and stack them in the ICC incubator. Take care with your technique because each groups plates will be shared with every group in the class. IS Each group will have 2 MS plates, Divide them in half and place the following samples on these plates: Nasal swab S. Epidermises S. erasures Vomits We Will use sterile cotton swabs for the nasal sample and vomits sample. Use your loop for the culture samples. Label your plates, invert them and stack them in the ICC incubator. 16. Each group will have a Blood Agar plate. Swab the back one students throat (sterile cotton swab) and transfer the sample using streak plating method to the blood agar plate. Class day 2: Look at the results of your different media plates. 17. In the space below, diagram your plate results. Label plates and color where appropriate, EMBED PEA MAC Blood MS 18 Pill in the following charts to help organize this information: Selects for. Important Bacteria among bacteria as to I hemolytic activity interconnect greenish/gray hue around I Differentiate by I Blood Agar I Color of agar Distinguishes I Clear zone around the I Streptococci and their ability to else Orbs. Bacteria, or I Embargo Distinguishes bacteria that ferment I Dark blue colonies with II. Oil and I Gram-negative bacteria lactose and or sucrose and those that green metallic sheen or organisms Did not. Pink. Imaginary For organisms that are I Isolates for imitation fermentation I Yellow color change in I Staphylococcus erasures I I I surrounding media, land Staphylococcus I Epidermises I halogenated. I McCracken Agar I Gram- negative bacteria. I Distinguished from lactose fermented Neutral red, and Interrogated arrogates, I produce a pink color land E. Coli, Epigram I bacteria or not I Isolation of obligate anaerobic I Distinguished from gram-negative and I transparent E. Oil and I bacteria I Staphylococcus erasures gram-positive bacteria. YOU WILL BE RESPONSE ABLE FOR THE FOLLOWING: o EMBED -? E. Coli recognition o Imitation Salt Stash recognition o Blood Agar Beta/Gamma hemolytic o PEA Gram (4) recognition o McCracken Gram G) recognition 19. Match the following plates with the above recognitions: [pick [pick] [pick [pick] [pick] A. McCracken Gram (-) recognition. 8. Blood Agar -Beta/Gamma hemolytic. Coli recognition. D. Imitation Salt Stash recognition. C. EMBED-E E. PEA Gram (+) recognition. Label-Medicaid Microbiology-Apart Tests for the Identification of Bacteria, Spasms Preparing for class Day 1 Read in your lab manual the following tests: Catalane Test (pug I SO), Oxides Test (pug 152), Coagulate Test (pug 166) to answer the following: 1. What do you remember (from lecture) about catalane? It is a common enzyme found in nearly all living organisms exposed to oxygen. 2. What is this enzyme involved in (from What we learned in lecture)? It catalysts the decomposition Of hydrogen peroxide o water and oxygen. 3. What does the Catalane Test test for? Is primarily used to distinguish among Gram-positive Cisco. 4. What does a positive Catalane Test result look like? Notable bubbling. What does a negative result look like? No bubbling. 5. What does the Oxides Test test for? To determine if bacteria have stockroom oxides, a participant in electron transport during respiration. 6. What is this enzyme involved in? Identification of bacterial strains: it determines whether a given bacterium produces stockroom oxides (and therefore utilizes oxygen with an electron transfer chain). 7. What does a positive Oxides Test result look like? Ill result in a color change to pink, through maroon and into black, within 10-30 seconds. What does a negative result look like? Will result in a light-pink or absence of coloration. 8, What does the Coagulate Test for? Pathogenic and non-pathogenic staphylococci. 9. What is this enzyme involved in? Staphylococcus erasures 10, Why is coagulate important to bacteria? Because of their ability to cause blood p lasma to clot 11. What does a coagulate positive result look like? Indicating by gelling of the plasma, which remains in place even after inverting the tube. What does a negative result look like? It flows when inverter 12. What bacteria are important in reference to the coagulate test? Staphylococcus erasures and Stash. Epidermis will demonstrate the Catalane, Oxides, and Coagulate tests. 13. On the box below, diagram the results Of the Catalane Test: Label results 14. In the box below, diagram the results of the Oxides Test. Label and use color where appropriate. IS In the box below, diagram the results of the Coagulate Test. Label and color where appropriate. 16. Fill in the following charts to help organize this information. Purpose Negative result Involved in I Positive Result I I Catalane Test TIT detect the presence tot I Quickly breakdown H2O into water and Bubbling I catalane, an enzyme that degrades 102 hydrogen peroxide I No Bubbling I I Oxides Test I Collects electrons and facilitates I Purple, maroon and into I Light pink or absent To determine if bacteria have I their addition to molecular 02 and black color color H2O during I respiration stockroom oxides, a participant I with to form line electron transport Coagulate Test TIT distinguish between pathogenic I Activates a pathway that converts I Gelling of the plasma, I Flows when inverted I and non-pathogenic staphylococci, forefinger in blood plasma into I remains in place even base on blood plasma clotting I fibrin, the protein thread sticks I after inverting the tube I forming clots Karen Hogan Label-Medical Microbiology part-3-Two Additional Tests for Identification of Bacteria: Latex Agglutination Test and Underwrote II Test Preparing tort class Day I Read the L atex Agglutination Test information provided and answer the following I. What does agglutination mean? Clumping of bacteria or red cells when held together by antibodies. 2. Since we are in microbiology are cooking for the clumping Of Epitomes found on the surface Of Antigen that Will bind to specific Antibody that were made by Immune system(B cells). 3. What Will a positive result look like? Clumping. 4. What will a negative result look like? Dilute liquid no clumping. Latex Agglutination Test The latex agglutination test is a laboratory method to check for certain antigens in a variety of bodily fluids including saliva, urine, cerebrations fluid, or blood. The sample is mixed with latex beads coated with a specific antibody. Fifth suspected substance is present (the specific antigen), the latex heads (with the pacific antibody) will clump together with the antigen (agglutinate). Antigen Antibody attached to beads in liquid When the antigen shape matches the antibody shape, they will bind to each other and the cells/antibody/antigen will clump together (as below). Notice how the dark spots are clumping in the liquid. When the antigen shape does not match the antibody shape, they will not bind to each other (see below). Notice that there are no clumps in the liquid. Procedure a) Place a drop of the Latex Control liquid in one of the circles on the test card. The Latex Control liquid will have the liquid contain the latex beads with no antibodies attached. B) Aseptically remove a colony from an agar plate and place it on the circle with the Control liquid. ) With the sterile loop, mix the liquid with the colony, d) Place a drop of the Latex Test liquid in the second circle on the test card The Latex Test liquid will have the liquid with antibodies for a specific microbe (in our class, the antigen is for Stash erasures) attached to the latex beads. E) Asepticall y remove a colony from an agar plate and place it in a second circle marked on the test card. F) With the sterile loop, mix the liquid with the colony. G) Compare the mixtures of the two colonies. 5. In the space below, diagram the results Of the Agglutination Test. Use color Preparing for class Day I Read the Underwrote II System information provided and answer the following: 1. What types of bacteria will the Underwrote II Test identity? E coli. 2. What information will the Underwrote II Test give us? ID gram-Eng, glucose fermenting, oases-negative intercontinental. The Underwrote II System The basic philosophy of the Underwrote II System is the speed, ease and low cost in the identification Of gram negative, glucose fermenting oxides-negative Intercontinental. The Underwrote II System consists of a single tube containing 2 compartment, each containing a different agar culture medium. There are compartments that require aerobic conditions and have small openings that allow air in; those compartments that require anaerobic conditions have a layer of paraffin wax on the top of the media. There is a self- enclosed inoculating needle or wire that runs through the center of the tube. The end of the needle can touch an isolated bacterial colony and then in one movement can he drawn through the 12 compartments so that every compartment is inoculated. [pick] After 18-24 hours of incubation, the color changes that occur in each of the impairments are recorded and interpreted according to the manufacturers instructions, The interpretation is done by determining a five-digit code from the results and then consulting a coding manual. [pick] Inoculating the tube: a. Remove the caps from both ends of the Underwrote. The tip of the wire is sterile and does not need to be flamed. B. Touch a well-isolated colony from an agar plate with the tip of the wire. C. Inoculate the Underwrote with the bacterial culture by drawing, and at the same time rotating, the wire through the 12 compartments. D. Push the ever back through the Underwrote so that the 12 hammers are re-inoculated. E. Withdraw the Wire once again until the tip is in the HAS/indolence compartment and then break the wire at the notch by bending back and forth. F. Replace the caps but do not tighten. Losing the Wire remnant, punch holes in the compartments that need to grow aerobically. G. Incubate the Underwrote for 18-24 hours at ICC. Interpreting the tube: a. After 18-24 hours of incubation, examine the Underwrote and notice the color changes that have occurred in each compartment. B. SE the color code chart provided in class to determine positive and negative results. C. Record both costive and negative results on the small worksheet provided during lab, d, We will skip the Indolence testing and the compartment labeled UP. E. Determine the five-digit identification number: 1. U se only the tests that are positive. Add the numbers under the results within each test section. 2. Enter the sum of the positive tests for each test section in the square labeled ID value. F. Determine the identity four enteric unknown by comparing the five- digit identification number with the Underwrote II Interpretation Guide (manual provided during lab), bacteria.