The Value Of Leadership In Oedipus Rex By Sophocles

Based on evidence, Athenians valued bravery, confidence and heroic strength in their leaders. For example, the entire reason Oedipus from Oedipus Rex by Sophocles became king of Thebes is because he defeated the Sphinx that was guarding the city, saving the citizens (info from the backstory). On page 11, a senator makes the comment, â€Å"if his bosom holds a grain of fear, curses like yours he never will abide.† The reader can assume the Athenians valued bravery because the senator makes a comment against the idea of fear. Also, Tiresias is mentioned to be â€Å"above all men,† which indicates that leaders need to be better than their inferiors (page 11). The king, the leader of Thebes, is referred to as â€Å"the mightiest head,† which signifies the†¦show more content†¦The outcome of dramatic impact is emphasized by the focus of one main subject. Oedipus attempts to escape him fate by running away, only to fulfill it (background information). Every ot her occurrence serves only to amplify the effect of the main subject. An example of this is the suicide of Jocasta, who hangs herself as a result of finding out she married her own son (page 44-45). This action emphasizes the fact that Oedipus attempted to avoid his fate, which made the consequences worse. In conclusion, rather than limiting the dramatic effect of the play, Aristotle’s three unities accentuate how the drama unfolds in the play and how it affects the listeners/readers. The whole idea of sight vs. blindness in Oedipus Rex points to the theme of fate and free will. For example, Tiresias points out that Oedipus â€Å"(has his) sight, and (does) not see,† referring to the fact that the king ignored his fate, instead, choosing to go out on his own (page 15). Oedipus is famously â€Å"blind† up until page 45, when he literally goes blind. Before he actually goes blind, the main character is â€Å"blind in (the) mind,† which means he is unable to see his fate that is right in front of him (page 14). By being blind to his own fate, Oedipus falls into the theme of illusion vs. reality. He lives in an illusion of perfection, being the king and married to a woman with whom he had four children (backstory). Reality hit Oedipus when it is made clear to him that he actuallyShow MoreRelatedKings as Main Characters in Literature Essay765 Words   |  4 Pagesto others. However, differences in culture, social system, values and social backgrou nd etc. actually endows heroes with diverse personalities varying from person to person and culture to culture. With rich imagination, every child molds his or her supreme leaders with authorities. But as time goes by, children are gradually matures, leaving their imagination during the childhood in the oblivious world, and their cognition of leadership also develops and changes without only confining to the worshipRead More Oedipus the King: Does Oedipus Satisfy the Definition of a Good Man?1415 Words   |  6 PagesDoes Oedipus Satisfy the Definition of a Good Man?      Ã‚  Ã‚  Ã‚   As a young man, Oedipus learned of his fate to kill his father and marry his mother.   Oedipus flees to a distant land to escape his terrible fate and inadvertently fulfills the prophecy. Unknowingly, Oedipus kills his father and enters the bed of his mother.   Was Oedipus was a good man who happened to suffer an unfortunate fate, or was he a truly bad person, whose fate was only just?   If we accept the Aristotelian views of good and badRead MoreCompare and Contrast Othello and Waiting for Godot Plays Essay1104 Words   |  5 Pages4/25/13 Oedipus the king is a representative of ancient Greek drama by Sophocles. The Latin title of the play is Oedipus Rex. Though produced as the second play in the three sequences of Theban plays by Sophocles, it finds its way at the top of the sequence as a result of internal chronology. The next play in the sequence is Oedipus at colonus and finally Antigone. The play is an excellent example of Greek tragic plays (Bloom, 51). 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Stigma and Prejudice in the Workplace - 844 Words

Workplace contributes strongly to the wellbeing of an individual. This is because it provides people with opportunities that make the feel productive and achieve their potentials. However, stigma and prejudice can make the workplace to be a very stressful and results into unwanted problems such as anxiety and depression (Reichert, 2011). Therefore, this paper aims at discussing how to create a safe workplace for stigmatized individuals. The paper describes various approaches that can be implemented to make workplace safe and free from stigmatization. Various approaches that can be used to create a safe workplace environment reduce stigmatization. The first one is to ensure that the workplace has inclusive environment and place that do not seclude or exclude stigmatized individuals. This is to prevent exposing the individuals to contradictory environments, which might identify them with the problem, challenge, or uniqueness (Saleebey, 2009). According to Szeto and Dobson (2011), obvious environment and places at work places, such as having separate offices for alcoholics, mentally challenged, or different social places for homosexuals may further enhance discrimination of the individuals. This implies that work place environment should be designed as inclusive as possible. Spagnolo, Murphy, Librera (2008) reported 80% of homosexual individuals find it easy to work in places, where they are not separated during social events, such break time sessions. Therefore, workplacesShow MoreRelatedLeadership Prejudices of Men and Women in t he Workplace Essay1592 Words   |  7 Pagesperspective to contrast the different leadership prejudices of men and women in the workplace. The age-old debate of inequality for women in executive position will now be examined through online periodicals that show gender stereotypes in the workforce, different strategies men and women use to break the glass ceiling of the workplace, a focus on the interactions and reactions of a male Chief Executive Officer (CEO) versus a female CEO, leadership prejudices among women and women, and various studiesRead MoreBeing Part Of A Stigmatized Group1287 Words   |  6 Pagessexual stigma to describe this phenomenon in relation to sexual minorities. Sexual stigma, in the researcher’s terms, means that there is an inferior status in relation to a society that does not tolerate of any nonheterosexual behavior, identity, or relationship (Herek Garnets, 2007). An example of this stigma would be a religion frowning upon a homosexual relatio nship and not tolerating or devaluing the homosexual couple. Herek also describes this at an individual level: sexual prejudice meansRead MoreChildren With Disabilities And Disabilities Essay1276 Words   |  6 Pages stigmatization, and prejudice among other forms of challenges. As such, it is possible to see many people with disabilities (e.g. visible: wheelchair bond or with cane and invisible: mental health or learning disabilities) are being socially and culturally affected by the general population’s perspective on them. This paper discusses stigmas, barriers, prejudices, and discrimination as the major difficulties that people with disabilities face in college and in the workplace along with policies andRead MoreEssay on Impact of Stigma of HIV-AIDS in the South African Workplace1497 Words   |  6 Pagesâ€Å"Impact of Stigma of HIV/AIDS in the South African workplace.† This falls under a discipline of business management in the financial management degree I am currently doing and one of the key areas of focus within business management is workplace challenges. I believe that HIV/AIDS stigmas are challenges regularly faced in the South African work environment. The purpose of this research review is to gain insight and deeper understanding of what workplace stigma towards HIV/AIDS is in the workplace fromRead MoreEffects On Psychological Health When Labelled With Chronic Illness1022 Words   |  5 PagesImpact on psychological health when labelled with chronic illness eg. stigma So my topic was how psychological health was affected when someone was labelled with a chronic illness. More specifically stigma associated with chronic illness and how it could impact Ivan due to his conditions. When someone is suffering or living with a chronic illness it can have a huge impact on them psychologically and socially. Chronic Illness is a condition that is prolonged in duration, usually more than 3 monthsRead MoreThe Stigma Of Invisible Disabilities. Invisible Disabilities1650 Words   |  7 PagesThe Stigma of Invisible Disabilities Invisible disabilities are exactly what they seem to be: hidden from view. Invisible disabilities come in almost endless varieties, including mental illnesses, HIV, AIDS, diabetes, learning disabilities, epilepsy, and more. While there may be visible components to these disabilities, all of them can be virtually invisible and â€Å"hidden† from view. People often make assumptions based on what is visibly seen, so when someone is suffering from something invisibleRead MoreAlicia Kate O. Borja. English 27 A. Andre Dominic Peralta.1260 Words   |  6 PagesAlicia Kate O. Borja English 27 A Andre Dominic Peralta Reducing Stigma in Mental Health Conditions Background on Mental Health Mental Health is often used as a representation of mental health conditions such as anxiety disorders, depression, schizophrenia, and others. 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Education is essential to our country, workplace and schools; we can learn from one another, but first we must have an understanding about ourselves as individuals. Social Workers bring unique knowledge and skills to the table and are instrumental in furthering the profession. Education to outside cultures and social groups can help social workers understand the differences within the world and helps dismiss negative stereotypes and prejudice around different groups. Education is key to understandingRead MoreThe Impact Of Workplace Diversity On The Work place997 Words   |  4 Pages Workplace diversity is a term which describes the inclusion of people in the workplace regardless of their cultural differences such as gender, race and sexuality. Rather than disregarding them, diverse workplaces utilise these differences to widen the range of experiences and skills among their employees as stated by the Australian Breastfeeding Association (2012). Some professions, however, are more diverse than others. According to the Diversity Inclusion: Unlocking Global Potential - Global

Physical Understanding System Development -Myassignmenthelp.Com

Question: Discuss About The Physical Understanding System Development? Answer: Introduction: The roadmap in the business change is considered as the high level and graphical overview of the project deliverables and goals represented through a timeline (Becker et al. 2012). The roadmap shows the changes in the business along with a plan to implement those changes. The changes in the business mainly happens due to implementation of information and communication technology or change in the business objectives. The report is based on the CC Musics software selection and implementation plan. The software selection process is done based on the objectives of the business process change. The project needs to be small and budget friendly. The recommendations is a great source of su9ggesting various implementation considerations. Current Situation of CC Music: CC Music is currently doing business with one Northern Soul band. The owners are experienced musicians and music technology teachers. As the owners have a long history associated with the bands, they have found it possible to conduct events and manage bands. The organization is responsible for managing studio works, TV and Radio programs and band performing on parties and events. The organization currently using a website to publicize the band. The booking process is done over the phone. The postal system is used for receiving cheque and contracts. All the processes of CC Music is done manually based on paper work. Only two members that are Chris and Clive has to do all the works on their own. Issues with the current situation: As the organization does not have any technology to assist in the business activities there are various issues in the organization. The paper based works are often tend to error and if a paper is lost then the data will have to be collected, unless a copy is present. As the organization is looking forward to grow its business, the biggest challenge will be maintaining all the paper based work and managing data. Chris and Clive will not be able to handle the huge amount of work if more bands are registered. The communication is a key factor in business growth. The organization does not have any proper way of storing the conversation. The communication among the external factors over the telephone will surely be a serious issue while the organization will handle multiple tasks simultaneously. The reports have to be generated manually which is always full of errors and affect the decision making process badly. Roadmap: Business Process Change Objectives: CC Music is willing to adopt a standard business approach to grow its business. The change must make the organization able to provide better and quality services to various bands. Better tracking of data is another objectives of business process change. Timeline: Process Start Date End Date Identify the software solution options 02/05/18 02/08/18 Compare and Select the software solution 02/09/18 02/10/18 Identify software development methodology options 02/12/18 02/13/18 Select the methodology 02/14/18 02/14/18 Employ the team members 02/15/18 02/19/18 Develop system 02/21/18 03/20/18 Train User 03/01/18 03/06/18 Proposed Changes: The main factor in the change of the business process is implementation or integration of ICT system in the environment of CC Music. The organization wants to provide services to several bands. In order to that CC Music must improve its infrastructure and business process. The most effective change in the process is communication. The organization will be integrating a software solution that will be consisting of website so that the clients and audiences can communicate with business anytime. The next big change is the change of paper based work into digital automation. The proposed software will store all the data into the system and these data will be used for creating report. Till now there is no decision making support to the owners of CC Music. Through the implementation of the IS, CC Music will introduce the decision making support to the business environment. Potential Risks: The change will be inviting various risks that has to be identified and mitigate. The risks of the change are wrong project estimation, cyber threats, improper training, internal threats, additional cost to implement change and wrong software selection. Risk Name Impact Likelihood Mitigation Strategies Wrong project estimation Medium High Risk avoidance Cyber threats Extreme Medium Risk transfer Improper training Medium Medium Risk avoidance Internal threats (Farkas et al. 2016) Medium Low Risk limitation Additional cost to implement change High High Risk acceptance Wrong software selection Extreme Medium Risk avoidance Selecting the Software Solution: The software selection is a crucial part of the project planning. There are four choices in the hands of Chris and Clive. They can either go with the open source ERP or implement a information system through open source language and database. In both the cases, the cloud system is preferable as it will reduce cost of hardware and other in-house development charges. As per the aforementioned objectives, a system that can cope with the business growth and provide a good opportunity to CC Music to increase client base is preferable. The PHP and MySQL based system is suggested to CC Music as this software solution will allow Chris and Clive to customize and change the software as per business requirements easily and with low expense. Process of Implementation: The process of implementation is followed by a planning phase where the SDLC methodology is selected. The traditional waterfall model is best suited for this software development project as the project is small and straight forward. The requirements of the software is well known and the user requirement collection procedure will be significantly small. After the planning is completed, the project team members will be hired (Laudon and Laudon 2016). Hiring employees is the only major expense in this project. The system requirements will be collected and evaluated in the next phase. After the requirements are identified, the system design will be executed. In this phase, the system interaction, behavior, data flow, database design and many more activities will be done. Based on the designs the next phase, development, will be carried out. The developed system will be continuously tested until a functioning system is generated (Putri and Rosa 2016). The tested system will be deployed in the environment of CC Music in the deployment phase. The users of the system will be provided training on how to use it during testing phase. Conclusion: Form the above report it can be concluded that the system is a crucial factor in the business process change. Chris and Clive needs to support the project from the beginning and communicating with other stakeholders is important part. The ERPNext could be selected for the organization but as Chris and Clive are looking for a long-term system that can support business objectives, the PHP and MySQL based system is perfect. The organization can later modify the systems each and every aspect as per their requirement. Recommendations: Skilled IT Professionals: Chris and Clive must hire skilled IT experts so that no issue can occur during system development. It has been seen that many SME has faced issues as the developers did not have enough knowledge and skill. Communication with Stakeholders: Communication among the project team and stakeholders of CC Music have a significant impact on the project. The IT system development is a critical task and often face unexpected challenges. In such situations, the stakeholders must support the project but it is recommended that communicating with the stakeholders on periodical basis helps in gaining their trust. References Alavi, M., 1984. An assessment of the prototyping approach to information systems development. Communications of the ACM, 27(6), pp.556-563. Becker, J., Delfmann, P., Eggert, M. and Schwittay, S., 2012. Generalizability and applicability of model-based business process compliance-checking approachesa state-of-the-art analysis and research roadmap. Business Research, 5(2), pp.221-247. Briggs, J.A., Wolvetang, E.J., Mattick, J.S., Rinn, J.L. and Barry, G., 2015. Mechanisms of long non-coding RNAs in mammalian nervous system development, plasticity, disease, and evolution. Neuron, 88(5), pp.861-877. Chavan, M.M. and Dongre, Y.V., 2015. MULTI-NODE DATABASE APPLICATION DEPLOYMENT ON LAMP ARCHITECTURE. International Journal of Advances in Engineering Technology, 7(6), p.1721. Delorme, A. and Makeig, S., 2004. EEGLAB: an open source toolbox for analysis of single-trial EEG dynamics including independent component analysis. Journal of neuroscience methods, 134(1), pp.9-21. Dolibarr, 2., January, N., DESCHAMPS, R., december), D., released, D., available, D. and CRM, D. 2018. Open Source ERP CRM software - Dolibarr. [online] Dolibarr.org. Available at: https://www.dolibarr.org/ [Accessed 4 Feb. 2018]. Erpnext.com. 2018. Open Source Cloud ERP - ERPNext. [online] Available at: https://erpnext.com/ [Accessed 4 Feb. 2018]. Farkas, D., Paz, E., Garrison, E., Greene, J., Gonzalez, J. and Zhao, K., 2016. A Research Inventory Database for the Hudson/Mohawk River Watershed Project. May-2016. https://csis. pace. edu/~ ctappert/srd2016/2016PDF/a7. pdf, accessed November. Garcia, C.M., Abilio, R. and Malheiros, N., 2015. Approaches and Technologies for Systems Integration: A Case Study at the Federal University of Lavras. Revista de Sistemas de Informao da FSMA, (15), pp.11-22. Garcia-Zapirain, B., de la Torre Dez, I., Sainz de Abajo, B. and Lpez-Coronado, M., 2016. Development, technical, and user evaluation of a web mobile application for self-control of diabetes. Telemedicine and e-Health, 22(9), pp.778-785. Heise, H., Crisan, A. and Theuvsen, L., 2015. The poultry market in Nigeria: market structures and potential for investment in the market. International Food and Agribusiness Management Review, 18, pp.197-222. Johansson, B. and Sudzina, F., 2008. ERP systems and open source: an initial review and some implications for SMEs. Journal of Enterprise Information Management, 21(6), pp.649-658. Laudon, K.C. and Laudon, J.P., 2016. Management information system. Pearson Education India. Liu, H., Jezek, K.C. and Li, B., 1999. Development of an Antarctic digital elevation model by integrating cartographic and remotely sensed data: a geographic information system based approach. Journal of Geophysical Research: Solid Earth, 104(B10), pp.23199-23213. Maruthi, R. and Jayakumari, C., 2014. SMS based bus tracking system using open source technologies. International Journal of Computer Applications, 86(9). Matthews, M.S., 2015. PHP and MYSQL Web Development: A Beginner's Guide. McGraw-Hill Education. Mejabi, O.V., Azeez, A.L., Adedoyin, A. and Oloyede, M.O., 2015, May. Challenges to Open Data Institutionalisation: Insights from stakeholder groups in Nigeria. In Open Data Research Symposium. Odoo S.A. 2018. Homepage. [online] Available at: https://www.odoo.com/ [Accessed 4 Feb. 2018]. Oon, R.D., 2010. Open Source ERP. ISBN-13, pp.978-9673490226. Putri, T.P. and Rosa, P.H.P., 2016. Decision Support System to Choose Digital Single Lens Camera with Simple Additive Weighting Method. Scientific Journal of Informatics, 3(2), pp.167-176. Rosemann, M. and vom Brocke, J., 2015. The six core elements of business process management. In Handbook on business process management 1 (pp. 105-122). Springer Berlin Heidelberg. Sherman, G.G., Lilian, R.R., Barron, P., Candy, S. and Bhardwaj, S., 2014. Laboratory information system data demonstrate successful implementation of the prevention of mother-to-child transmission programme in South Africa: prevention of mother-to-child transmission-Progress towards the Millennium Development Goals. South African Medical Journal, 104(3), pp.235-238. Smets-Solanes, J.P. and De Carvalho, R.A., 2003. ERP5: a next-generation, open-source ERP architecture. IT professional, 5(4), pp.38-44. Tor, I.E., Iheanacho, A.C. and Okeke, A.M., 2017. Analysis of Socioeconomic Challenges of Using Local Storage Systems for Root and Tuber Crops in Benue State, Nigeria. Vidgen, R., 2002. Constructing a web information system development methodology. Information systems journal, 12(3), pp.247-261. Yuan, X., Wood, E.F. and Ma, Z., 2015. A review on climate?model?based seasonal hydrologic forecasting: physical understanding and system development. Wiley Interdisciplinary Reviews: Water, 2(5), pp.523-536.

Microbiology Unknown Lab Report Essay Example

Microbiology Unknown Lab Report Paper Preparing for class Day I Read in your lab manual about the following agar mediums: Blood Agar (pug 168), EMBED Agar (pug 170), Imitation Salt Agar 172)), McCracken Agar (pug 174), ND PEA Agar (pug 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to else red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them? Beta hemolytic, which is the complete lysine of red blood cells and hemoglobin. This results in complete clearing tooth blood around the colonies. Alpha hemolytic refers to the partial lysine of red blood cells and hemoglobin, This results in a greenish-grey disconsolation tot the blood around the colonies. Employees, sometimes called gamma hemolytic results in no change in the medium. 3. What color is the EMBED agar? Dark blue colonies with green metallic sheen or pink. 4, What does the EMBED agar select for? Cram-negative bacteria. 5. What bacteria can easily be differentiated on EMBED agar? Gram-positive. How is it recognized? It contains the dyes eosin and methyl blue, which inhibit the growth of gram-positive bacteria. 6. What color is the Imitation Salt Agar (MS)? Yellow color change in surrounding media. 7. What does the MS agar select for? It contains 7. 5% sodium chloride, which selects for organisms that re halogenated. 8. Which bacteria (that we have learned about in lecture) can be seen on MS agar? The organism Staphylococcus erasures. How can it be recognized? Capable Of imitation fermentation, signified by the color change in the surrounding media. 9. What color is McCracken Agar? We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Microbiology Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Neutral red, and produce a pink color. 10. What does the McCracken agar select for? Gram. Negative bacteria. 11. What color is PEA agar? Small transparent colonies 12. What does the PEA agar select for? Isolation of obligate anaerobic bacteria. Class day 1 14 We will be setting up the Selective/Differential Media plates for each group s follows: Each group will have one bacteria sample and will do streak plates on each of the 3 agar plates (EMBED, PEA and McCracken). Label the bottom of the plate with your culture name. Invert your plates and stack them in the ICC incubator. Take care with your technique because each groups plates will be shared with every group in the class. IS Each group will have 2 MS plates, Divide them in half and place the following samples on these plates: Nasal swab S. Epidermises S. erasures Vomits We Will use sterile cotton swabs for the nasal sample and vomits sample. Use your loop for the culture samples. Label your plates, invert them and stack them in the ICC incubator. 16. Each group will have a Blood Agar plate. Swab the back one students throat (sterile cotton swab) and transfer the sample using streak plating method to the blood agar plate. Class day 2: Look at the results of your different media plates. 17. In the space below, diagram your plate results. Label plates and color where appropriate, EMBED PEA MAC Blood MS 18 Pill in the following charts to help organize this information: Selects for. Important Bacteria among bacteria as to I hemolytic activity interconnect greenish/gray hue around I Differentiate by I Blood Agar I Color of agar Distinguishes I Clear zone around the I Streptococci and their ability to else Orbs. Bacteria, or I Embargo Distinguishes bacteria that ferment I Dark blue colonies with II. Oil and I Gram-negative bacteria lactose and or sucrose and those that green metallic sheen or organisms Did not. Pink. Imaginary For organisms that are I Isolates for imitation fermentation I Yellow color change in I Staphylococcus erasures I I I surrounding media, land Staphylococcus I Epidermises I halogenated. I McCracken Agar I Gram- negative bacteria. I Distinguished from lactose fermented Neutral red, and Interrogated arrogates, I produce a pink color land E. Coli, Epigram I bacteria or not I Isolation of obligate anaerobic I Distinguished from gram-negative and I transparent E. Oil and I bacteria I Staphylococcus erasures gram-positive bacteria. YOU WILL BE RESPONSE ABLE FOR THE FOLLOWING: o EMBED -? E. Coli recognition o Imitation Salt Stash recognition o Blood Agar Beta/Gamma hemolytic o PEA Gram (4) recognition o McCracken Gram G) recognition 19. Match the following plates with the above recognitions: [pick [pick] [pick [pick] [pick] A. McCracken Gram (-) recognition. 8. Blood Agar -Beta/Gamma hemolytic. Coli recognition. D. Imitation Salt Stash recognition. C. EMBED-E E. PEA Gram (+) recognition. Label-Medicaid Microbiology-Apart Tests for the Identification of Bacteria, Spasms Preparing for class Day 1 Read in your lab manual the following tests: Catalane Test (pug I SO), Oxides Test (pug 152), Coagulate Test (pug 166) to answer the following: 1. What do you remember (from lecture) about catalane? It is a common enzyme found in nearly all living organisms exposed to oxygen. 2. What is this enzyme involved in (from What we learned in lecture)? It catalysts the decomposition Of hydrogen peroxide o water and oxygen. 3. What does the Catalane Test test for? Is primarily used to distinguish among Gram-positive Cisco. 4. What does a positive Catalane Test result look like? Notable bubbling. What does a negative result look like? No bubbling. 5. What does the Oxides Test test for? To determine if bacteria have stockroom oxides, a participant in electron transport during respiration. 6. What is this enzyme involved in? Identification of bacterial strains: it determines whether a given bacterium produces stockroom oxides (and therefore utilizes oxygen with an electron transfer chain). 7. What does a positive Oxides Test result look like? Ill result in a color change to pink, through maroon and into black, within 10-30 seconds. What does a negative result look like? Will result in a light-pink or absence of coloration. 8, What does the Coagulate Test for? Pathogenic and non-pathogenic staphylococci. 9. What is this enzyme involved in? Staphylococcus erasures 10, Why is coagulate important to bacteria? Because of their ability to cause blood p lasma to clot 11. What does a coagulate positive result look like? Indicating by gelling of the plasma, which remains in place even after inverting the tube. What does a negative result look like? It flows when inverter 12. What bacteria are important in reference to the coagulate test? Staphylococcus erasures and Stash. Epidermis will demonstrate the Catalane, Oxides, and Coagulate tests. 13. On the box below, diagram the results Of the Catalane Test: Label results 14. In the box below, diagram the results of the Oxides Test. Label and use color where appropriate. IS In the box below, diagram the results of the Coagulate Test. Label and color where appropriate. 16. Fill in the following charts to help organize this information. Purpose Negative result Involved in I Positive Result I I Catalane Test TIT detect the presence tot I Quickly breakdown H2O into water and Bubbling I catalane, an enzyme that degrades 102 hydrogen peroxide I No Bubbling I I Oxides Test I Collects electrons and facilitates I Purple, maroon and into I Light pink or absent To determine if bacteria have I their addition to molecular 02 and black color color H2O during I respiration stockroom oxides, a participant I with to form line electron transport Coagulate Test TIT distinguish between pathogenic I Activates a pathway that converts I Gelling of the plasma, I Flows when inverted I and non-pathogenic staphylococci, forefinger in blood plasma into I remains in place even base on blood plasma clotting I fibrin, the protein thread sticks I after inverting the tube I forming clots Karen Hogan Label-Medical Microbiology part-3-Two Additional Tests for Identification of Bacteria: Latex Agglutination Test and Underwrote II Test Preparing tort class Day I Read the L atex Agglutination Test information provided and answer the following I. What does agglutination mean? Clumping of bacteria or red cells when held together by antibodies. 2. Since we are in microbiology are cooking for the clumping Of Epitomes found on the surface Of Antigen that Will bind to specific Antibody that were made by Immune system(B cells). 3. What Will a positive result look like? Clumping. 4. What will a negative result look like? Dilute liquid no clumping. Latex Agglutination Test The latex agglutination test is a laboratory method to check for certain antigens in a variety of bodily fluids including saliva, urine, cerebrations fluid, or blood. The sample is mixed with latex beads coated with a specific antibody. Fifth suspected substance is present (the specific antigen), the latex heads (with the pacific antibody) will clump together with the antigen (agglutinate). Antigen Antibody attached to beads in liquid When the antigen shape matches the antibody shape, they will bind to each other and the cells/antibody/antigen will clump together (as below). Notice how the dark spots are clumping in the liquid. When the antigen shape does not match the antibody shape, they will not bind to each other (see below). Notice that there are no clumps in the liquid. Procedure a) Place a drop of the Latex Control liquid in one of the circles on the test card. The Latex Control liquid will have the liquid contain the latex beads with no antibodies attached. B) Aseptically remove a colony from an agar plate and place it on the circle with the Control liquid. ) With the sterile loop, mix the liquid with the colony, d) Place a drop of the Latex Test liquid in the second circle on the test card The Latex Test liquid will have the liquid with antibodies for a specific microbe (in our class, the antigen is for Stash erasures) attached to the latex beads. E) Asepticall y remove a colony from an agar plate and place it in a second circle marked on the test card. F) With the sterile loop, mix the liquid with the colony. G) Compare the mixtures of the two colonies. 5. In the space below, diagram the results Of the Agglutination Test. Use color Preparing for class Day I Read the Underwrote II System information provided and answer the following: 1. What types of bacteria will the Underwrote II Test identity? E coli. 2. What information will the Underwrote II Test give us? ID gram-Eng, glucose fermenting, oases-negative intercontinental. The Underwrote II System The basic philosophy of the Underwrote II System is the speed, ease and low cost in the identification Of gram negative, glucose fermenting oxides-negative Intercontinental. The Underwrote II System consists of a single tube containing 2 compartment, each containing a different agar culture medium. There are compartments that require aerobic conditions and have small openings that allow air in; those compartments that require anaerobic conditions have a layer of paraffin wax on the top of the media. There is a self- enclosed inoculating needle or wire that runs through the center of the tube. The end of the needle can touch an isolated bacterial colony and then in one movement can he drawn through the 12 compartments so that every compartment is inoculated. [pick] After 18-24 hours of incubation, the color changes that occur in each of the impairments are recorded and interpreted according to the manufacturers instructions, The interpretation is done by determining a five-digit code from the results and then consulting a coding manual. [pick] Inoculating the tube: a. Remove the caps from both ends of the Underwrote. The tip of the wire is sterile and does not need to be flamed. B. Touch a well-isolated colony from an agar plate with the tip of the wire. C. Inoculate the Underwrote with the bacterial culture by drawing, and at the same time rotating, the wire through the 12 compartments. D. Push the ever back through the Underwrote so that the 12 hammers are re-inoculated. E. Withdraw the Wire once again until the tip is in the HAS/indolence compartment and then break the wire at the notch by bending back and forth. F. Replace the caps but do not tighten. Losing the Wire remnant, punch holes in the compartments that need to grow aerobically. G. Incubate the Underwrote for 18-24 hours at ICC. Interpreting the tube: a. After 18-24 hours of incubation, examine the Underwrote and notice the color changes that have occurred in each compartment. B. SE the color code chart provided in class to determine positive and negative results. C. Record both costive and negative results on the small worksheet provided during lab, d, We will skip the Indolence testing and the compartment labeled UP. E. Determine the five-digit identification number: 1. U se only the tests that are positive. Add the numbers under the results within each test section. 2. Enter the sum of the positive tests for each test section in the square labeled ID value. F. Determine the identity four enteric unknown by comparing the five- digit identification number with the Underwrote II Interpretation Guide (manual provided during lab), bacteria.